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Elisa evaluation of the levels of antibodies against Infectious Bronchitis Virus in laying hens using egg yolk as substrate Rev. Bras. Ciênc. Avic.
Rauber,RH; Flôres,ML; Pereira,CE; Grigulo,M.
The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibody; Egg yolk; ELISA; Infectious bronchitis; Serology.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200008
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Interference of Salmonella typhimurium lipopolysaccharide on performance and biological parameters of broiler chickens Rev. Bras. Ciênc. Avic.
Rauber,RH; Perlin,VJ; Fin,CD; Mallmann,AL; Miranda,DP; Giacomini,LZ; Nascimento,VP do.
This study was conducted to determine the interference of Salmonella typhimurium lipopolysaccharide (sLPS) on the performance, biological parameters, and histological evaluations of 198 one-day-old male broiler chickens divided into three treatments according to sLPS dose (0, 250, or 500 µg/application/bird) that was applied to the birds every other day, from 15 to 27 days of age. At the end of the experiment (28 days), significant effects were observed on body weight (R= -0.17 and P=0.05), total cholesterol serum levels(R=0.43 and p<0.01), phosphorus (R=0.53 and P<0.01), uric acid (R= -0.38 and P<0.01), C-reactive protein (R=0.68 and p<0.01), serum activity of aspartate aminotransferase (R=0.39 and p<0.01) and alkaline phosphatase (R= -0.39...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poultry; Clinical biochemistry; Endotoxin; Body weight.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100011
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